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生物工具
格式轉(zhuǎn)換工具

合并FASTA格式序列

EMBL格式轉(zhuǎn)換為FASTA格式

EMBL特征抽取器

EMBL翻譯信息提取

DNA序列清理

蛋白序列清理

GenBank格式轉(zhuǎn)換成FASTA格式

GenBank文件特征提取器

GenBank翻譯信息提取

氨基酸單字母到三字母

DNA子串提取

蛋白序列子串提取

反向互補(bǔ)序列計(jì)算

密碼子分離器

FASTA文件分割

氨基酸三字母到單字母

滑動(dòng)窗口方式提取DNA子串

滑動(dòng)窗口方式提取蛋白序列子串
序列分析工具一

繪制密碼子譜

密碼子使用頻率表計(jì)算

CpG島分析

DNA分子量計(jì)算

正則表達(dá)式查找DNA子串

DNA堿基統(tǒng)計(jì)

模糊方式獲取DNA子串

模糊方式獲取蛋白子串

DNA/蛋白序列相似度比較

根據(jù)已知蛋白序列生成cDNA

酶切突變工具

開放閱讀框查找

雙序列密碼子比對(duì)

雙序列DNA堿基比對(duì)

雙序列蛋白殘基比對(duì)
序列分析工具二

PCR引物信息統(tǒng)計(jì)

PCR產(chǎn)物推測(cè)

蛋白親水分析工具

蛋白等電點(diǎn)計(jì)算器

蛋白分子量計(jì)算

正則表達(dá)式查找蛋白子串

蛋白序列氨基酸組成統(tǒng)計(jì)

DNA模擬酶切分析

常見酶切位點(diǎn)統(tǒng)計(jì)

蛋白序列到基因序列

基因序列到蛋白序列
序列圖形化分析工具

顏色法標(biāo)記DNA/蛋白保守區(qū)

顏色法標(biāo)記蛋白殘基生化相似度

DNA帶數(shù)字分組格式化

蛋白序列帶數(shù)字分組格式化

模板引物匹配圖

酶切位點(diǎn)標(biāo)記圖

DNA翻譯圖譜
隨機(jī)序列生成工具

隨機(jī)突變DNA

隨機(jī)突變蛋白序列

隨機(jī)生成cDNA

隨機(jī)生成DNA

隨機(jī)突變特定位置的DNA

隨機(jī)生成蛋白質(zhì)序列

隨機(jī)突變蛋白序列的特定位置

DNA隨機(jī)采樣

蛋白質(zhì)隨機(jī)采樣

打亂DNA

打亂蛋白序列
Miscellaneous

IUPAC編碼表

密碼子表

瀏覽器兼容性要求

如何鏡像此程序

單機(jī)版使用方法

關(guān)于序列操作套件

致謝

文獻(xiàn)

EMBL Feature提取器

EMBL特征提取器(EMBL Feature Extractor)可以依照EMBL中Feature的定義信息，將DNA片段提取并拼接出FASTA格式。在需要從基因組中去除內(nèi)含子、獲取cDNA時(shí)，此工具特別有用。

粘貼一條或多條EMBL文件內(nèi)容，長度限定在200000以內(nèi)。

ID   AF177870   standard; DNA; INV; 3123 BP.
XX
AC   AF177870;
XX
SV   AF177870.1
XX
DT   02-NOV-1999 (Rel. 61, Created)
DT   17-AUG-2000 (Rel. 64, Last updated, Version 2)
XX
DE   Caenorhabditis sp. CB5161 putative PP2C protein phosphatase FEM-2 (fem-2)
DE   gene, complete cds.
XX
KW   .
XX
OS   Caenorhabditis sp. CB5161
OC   Eukaryota; Metazoa; Nematoda; Chromadorea; Rhabditida; Rhabditoidea;
OC   Rhabditidae; Peloderinae; Caenorhabditis.
XX
RN   [1]
RP   1-3123
RA   Stothard P.M., Hansen D., Pilgrim D.;
RT   "Isolation of PP2C sequences using degenerate-oligo PCR";
RL   Unpublished.
XX
RN   [2]
RP   1-3123
RA   Stothard P.M., Hansen D., Pilgrim D.;
RT   ;
RL   Submitted (17-AUG-1999) to the EMBL/GenBank/DDBJ databases.
RL   Biological Sciences, University of Alberta, Edmonton, AB T6G-2E9, Canada
XX
DR   SPTREMBL; Q9U6S2; Q9U6S2.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..3123
FT                   /db_xref="taxon:135651"
FT                   /organism="Caenorhabditis sp. CB5161"
FT                   /strain="CB5161"
FT   mRNA            join(<265..402,673..781,911..1007,1088..1215,1377..1573,
FT                   1866..2146,2306..2634,2683..>2855)
FT                   /gene="fem-2"
FT                   /product="putative FEM-2 protein phosphatase type 2C"
FT   CDS             join(265..402,673..781,911..1007,1088..1215,1377..1573,
FT                   1866..2146,2306..2634,2683..2855)
FT                   /codon_start=1
FT                   /db_xref="SPTREMBL:Q9U6S2"
FT                   /note="possible sex-determining protein"
FT                   /gene="fem-2"
FT                   /product="putative PP2C protein phosphatase FEM-2"
FT                   /protein_id="AAF04557.1"
FT                   /translation="MSDSLNHPSSSTVHADDGFEPPTSPEDNNKKPSLEQIKQEREALF
FT                   TDLFADRRRSARSVIEEAFQNELMSAEPVQPNVPNPHSIPIRFRHQPVAGPAHDVFGDA
FT                   VHSIFQKIMSRGVNADYSHWMSYWIALGIDKKTQMNYHMKPFCKDTYATEGSLEAKQTF
FT                   TDKIRSAVEEIIWKSAEYCDILSEKWTGIHVSADQLKGQRNKQEDRFVAYPNGQYMNRG
FT                   QSDISLLAVFDGHGGHECSQYAAAHFWEAWSDAQHHHSQDMKLDELLEKALETLDERMT
FT                   VRSVRESWKGGTTAVCCAVDLNTNQIAFAWLGDSPGYIMSNLEFRKFTTEHSPSDPEEC
FT                   RRVEEVGGQIFVIGGELRVNGVLNLTRALGDVPGRPMISNKPDTLLKTIEPADYLVLLA
FT                   CDGISDVFNTSDLYNLVQAFVNEYDVEDYHELARYICNQAVSAGSADNVTVVIGFLRPP
FT                   EDVWRVMKTDSDDEESELEEEDDNE"
XX
SQ   Sequence 3123 BP; 986 A; 605 C; 597 G; 935 T; 0 other;
     gaacgcgaat gcctctctct ctttcgatgg gtatgccaat tgtccacatt cactcgtgtt        60
     gcctcctctt tgccaacacg caagacacca gaaacgcgtc aaccaaagag aaaaagacgc       120
     cgacaacggg cagcactcgc gagagacaaa ggttatcgcg ttgtgttatt atacattcgc       180
     atccgggtca actttagtcc gttgaacatg cttcttgaaa acctagttct cttaaaataa       240
     cgttttagaa gttttggtct tcagatgtct gattcgctaa atcatccatc gagttctacg       300
     gtgcatgcag atgatggatt cgagccacca acatctccgg aagacaacaa caaaaaaccg       360
     tctttagaac aaattaaaca ggaaagagaa gcgttgttta cggttagtta cctattagct       420
     gcaagttttg aaaaagcgga atctgtaaaa agcggaatct gtaaaaaaaa catctaagga       480
     ataattctga aaagaaaaag tttctaaatg ttaatcggaa tccaattttt atgaaattat       540
     ttaaaaaaaa actaaaatta gtttctaaaa aatttttcta aagtaattgg accatgtgaa       600
     ggtacaccca cttgttccaa tatgccatat ctaactgtaa aataatttga ttctcatgag       660
     aatatttttc aggatctatt cgcagatcgt cgacgaagcg ctcgttctgt gattgaagaa       720
     gctttccaaa acgaactcat gagtgctgaa ccagtccagc caaacgtgcc gaatccacat       780
     tgtgagttgg aaatttttat ttgataacca agagaaaaaa agttctacct ttttttcaaa       840
     aacctttcca aaaatgattc catctgatat aggattaaga aaaatatttt ccgaaatctc       900
     tgcttttcag cgattcccat tcgtttccgt catcaaccag ttgctggacc tgctcatgat       960
     gttttcggag acgcggtgca ttcaattttt caaaaaataa tgtccaggta tacactattt      1020
     ttgcatattt ttcttgccaa atttggtcaa aaaccgtagt acaacccaaa aagtttcttc      1080
     atttcagagg agtgaacgcg gattatagtc attggatgtc atattggatc gcgttgggaa      1140
     tcgacaaaaa aacacaaatg aactatcata tgaaaccgtt ttgcaaagat acttatgcaa      1200
     ctgaaggctc cttaggtagg ttagtctttt ctaggcacag aagagtgaga aaattctaaa      1260
     tttctgagca gtctgctttt tgttttcctt gagtttttac ttaaagctct taaaagaaat      1320
     ctaggcgtga agttcgagcc ttgtaccata ccacaacagc attccaaatg ttacagaagc      1380
     gaaacaaaca tttactgata aaatcaggtc agctgttgag gaaattatct ggaagtccgc      1440
     tgaatattgt gatattctta gcgagaagtg gacaggaatt catgtgtcgg ccgaccaact      1500
     gaaaggtcaa agaaataagc aagaagatcg ttttgtggct tatccaaatg gacaatacat      1560
     gaatcgtgga caggttagtg cgaatcgggg actcaagatt tactgaaata gtgaagagaa      1620
     aacaaaagaa aactatattt tcaaaaaaaa tgagaactct aataaacaga atgaaaaaca      1680
     ttcaaagcta cagtagtatt tccagctgga gtttccagag ccaaaaaaat gcgagtatta      1740
     ctgtagtttt gaaattggtt tctcacttta cgtacgattt tttgattttt ttttcagact      1800
     cttcatatga aaaaaaatca tgttttctcc tttacaagat ttttttgatc tcaaaacatt      1860
     tccagagtga catttcactt cttgcggtgt tcgatgggca tggcggacac gagtgctctc      1920
     aatatgcagc tgctcatttc tgggaagcat ggtccgatgc tcaacatcat cattcacaag      1980
     atatgaaact tgacgaactc ctagaaaagg ctctagaaac attggacgaa agaatgacag      2040
     tcagaagtgt tcgagaatct tggaaaggtg gaaccactgc tgtctgctgt gctgttgatt      2100
     tgaacactaa tcaaatcgca tttgcctggc ttggagattc accagggtaa tcaatttttt      2160
     tttagttttt ggaactttac gtcccgaaaa attattcctt tatcacctaa ttcctacagt      2220
     aacccaagct ccgaattaaa taaagttaaa gcgtggtata cacataaaaa taagaaaaaa      2280
     ttgttcatga aatccatttt tccagttaca tcatgtcaaa cttggagttc cgcaaattca      2340
     ctactgaaca ctccccgtct gacccggagg aatgtcgacg agtcgaagaa gtcggtggcc      2400
     agatttttgt gatcggtggt gagctccgtg tgaatggagt actcaacctg acgcgagcac      2460
     taggagacgt acctggaaga ccaatgatat ccaacaaacc tgatacctta ctgaagacga      2520
     tcgaacctgc ggattatctt gttttgttgg cctgtgacgg gatttctgac gtcttcaaca      2580
     ctagtgattt gtacaatttg gttcaggctt ttgtcaatga atatgacgta gaaggtatca      2640
     aactgatcgt ttttcacatc acaaaattct tgaattttcc agattatcac gaacttgcac      2700
     gctacatttg caatcaagca gtttcagctg gaagtgctga caatgtgaca gtagttatag      2760
     gtttcctccg tccaccagaa gacgtttggc gtgtaatgaa aacagactcg gatgatgaag      2820
     agagcgagct cgaggaagaa gatgacaatg aatagtttat tgcaagtttt ccaaaacttt      2880
     tccaatttcc ctgggtattg attagcatcc atatcttacg gcgattatat caattgtaac      2940
     attatttctg tttctccccc cacctctcaa attttcaaat gacccttttt cttttcgtct      3000
     acctgtatcg ttttccattc atctcccccc ctccactgtg gtatatcatt ttgtcattag      3060
     aaagtattat tttgattttc attggcagta gaagacaaca ggatacagaa gaggttttca      3120
     cag                                                                    3123
//

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